| First Author | Sakai I | Year | 1987 |
| Journal | Biochem J | Volume | 242 |
| Issue | 2 | Pages | 619-22 |
| PubMed ID | 2439071 | Mgi Jnum | J:4448 |
| Mgi Id | MGI:57205 | Doi | 10.1042/bj2420619 |
| Citation | Sakai I, et al. (1987) Molecular cloning and nucleotide sequence of the cDNA for sperm-specific lactate dehydrogenase-C from mouse. Biochem J 242(2):619-22 |
| abstractText | Mouse sperm-specific lactate dehydrogenase-C (LDH-C) cDNA was cloned and sequenced from lambda gt11 expression library. The LDH-C cDNA insert of 1236 bp consists of the protein-coding sequence (999 bp), the 5' (54 bp) and 3' (113 bp) non-coding regions, and the poly(A) tail (70 bp). The Northern blot analysis of poly(A)-containing RNAs from mouse testes and liver indicates that the LDH-C gene is expressed in testes but not in liver, and that its mRNA is approx. 1400 nucleotides in length. The nucleotide and amino acid sequences of the mouse LDH-C cDNA show 73% and 72% homologies, respectively, with those of the mouse LDH-A. The Southern blot analysis of genomic DNAs from mouse liver and human placenta indicates the presence of multiple LDH-C gene-related sequences. |
