| First Author | McNally JG | Year | 2000 |
| Journal | Science | Volume | 287 |
| Issue | 5456 | Pages | 1262-5 |
| PubMed ID | 10678832 | Mgi Jnum | J:60637 |
| Mgi Id | MGI:1353748 | Doi | 10.1126/science.287.5456.1262 |
| Citation | McNally JG, et al. (2000) The glucocorticoid receptor: rapid exchange with regulatory sites in living cells. Science 287(5456):1262-5 |
| abstractText | Steroid receptors bind to site-specific response elements in chromatin and modulate gene expression in a hormone-dependent fashion. With the use of a tandem array of mouse mammary tumor virus reporter elements and a form of glucocorticoid receptor labeled with green fluorescent protein, targeting of the receptor to response elements in live mouse cells was observed. Photobleaching experiments provide direct evidence that the hormone-occupied receptor undergoes rapid exchange between chromatin and the nucleoplasmic compartment. Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed. |
