| First Author | Aizawa Y | Year | 1999 |
| Journal | FEBS Lett | Volume | 445 |
| Issue | 2-3 | Pages | 338-42 |
| PubMed ID | 10094485 | Mgi Jnum | J:53429 |
| Mgi Id | MGI:1332718 | Doi | 10.1016/s0014-5793(99)00148-9 |
| Citation | Aizawa Y, et al. (1999) Cloning and expression of interleukin-18 binding protein. FEBS Lett 445(2-3):338-42 |
| abstractText | Interleukin-18 binding protein is a novel glycoprotein that we successfully cloned and expressed. First, murine interleukin-18 binding protein was purified from the sera of mice with endotoxin shock using ligand affinity chromatography. The murine interleukin-18 binding protein cDNA was cloned after RT-PCR using mixed primer pair sequences based on partial murine interleukin-18 binding protein amino acid sequence analysis. Subsequently, human interleukin-18 binding protein cDNA was cloned from cDNA libraries of normal human liver using murine interleukin-18 binding protein cDNA as a probe. Next, we transiently expressed recombinant human and murine interleukin-18 binding proteins in COS-1 cells and purified them from culture supernatants. Both recombinant interleukin-18 binding proteins did not exhibit species specificity and prevented interleukin-18 binding to its receptor. In addition, they inhibited interleukine-18 dependent IFN-gamma production from KG-1 cells effectively. These results suggest that the interleukin-18 binding protein may possess interleukine-18 antagonist activity. |
