| First Author | van Maanen M | Year | 1999 |
| Journal | Biochim Biophys Acta | Volume | 1447 |
| Issue | 2-3 | Pages | 284-90 |
| PubMed ID | 10542328 | Mgi Jnum | J:58254 |
| Mgi Id | MGI:1347139 | Doi | 10.1016/s0167-4781(99)00159-1 |
| Citation | van Maanen M, et al. (1999) Characterization of mouse glycogenin-1 cDNA and promoter region. Biochim Biophys Acta 1447(2-3):284-90 |
| abstractText | Glycogenin-1 is an autocatalytic, self-glucosylating protein which acts as the primer for glycogen synthesis in mammalian skeletal muscle. In this study, we have cloned the glycogenin-1 cDNA from mouse skeletal muscle. Mouse glycogenin-1 has a predicted molecular mass of 37 omitted399 Da, and the deduced amino acid sequence exhibited 87% homology with human glycogenin-1. Northern blot analysis specifically detected mouse glycogenin-1 transcript in skeletal muscle and heart, and to a lesser extent in kidney, lung and brain. 5'-RACE analysis revealed the major transcription start site to be localized 47 bp upstream of the initiation of translation codon. Sequence analysis of approximately 2 kb of the 5'-flanking region revealed potentially important regions of homology between the mouse and human glycogenin-1 promoters. Several conserved but putative elements, including a TATA box, Sp1 site, and a cyclic AMP responsive element, were observed proximal to the transcription start site. Significantly, Northern blot analysis revealed dibutyryl-cAMP treatment of cultured mouse C2C12 myotubes markedly reduced the levels of glycogenin-1 mRNA in a dose-dependent manner. |
