| First Author | Gordon C | Year | 1993 |
| Journal | Nature | Volume | 366 |
| Issue | 6453 | Pages | 355-7 |
| PubMed ID | 8247131 | Mgi Jnum | J:40216 |
| Mgi Id | MGI:87558 | Doi | 10.1038/366355a0 |
| Citation | Gordon C, et al. (1993) Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit. Nature 366(6453):355-7 |
| abstractText | We have isolated a mutant, mts2, in the fission yeast Schizosaccharomyces pombe which is defective in chromosome segregation. The predicted amino-acid sequence of the cloned mts2+ gene product is 75% identical to the S4 subunit of the human 26S ATP/ubiquitin-dependent protease. The human S4 subunit complementary DNA expressed from an S. pombe expression plasmid can rescue an S. pombe mts2 gene disruption. Both observations demonstrate that the mts2+ gene is the S. pombe homologue of the human S4 subunit. In addition, we provide genetic evidence for a physical interaction between the S4 and the related S7 subunit in the 26S multiprotein protease. We show that polyubiquitin-conjugated proteins accumulate in the mts2 mutant at the restrictive temperature, demonstrating that the mutant has an in vivo defect in the ubiquitin-dependent proteolysis pathway. Finally, the phenotype for the mts2 mutant indicates that protein degradation by the 26S protease is essential not for entry into but for the completion of mitosis. |
