| First Author | Driessen CA | Year | 1998 |
| Journal | FEBS Lett | Volume | 428 |
| Issue | 3 | Pages | 135-40 |
| PubMed ID | 9654122 | Mgi Jnum | J:46752 |
| Mgi Id | MGI:1271037 | Doi | 10.1016/s0014-5793(98)00473-6 |
| Citation | Driessen CA, et al. (1998) The visual cycle retinol dehydrogenase: possible involvement in the 9-cis retinoic acid biosynthetic pathway. FEBS Lett 428(3):135-40 |
| abstractText | The 11-cis-retinol dehydrogenase (11-cis-RoDH) gene encodes the short-chain alcohol dehydrogenase responsible for 11-cis-retinol oxidation in the visual cycle. The structure of the murine 11-cis-RoDH gene was used to reinvestigate its transcription pattern. An 11-cis-RoDH gene transcript was detected in several non-ocular tissues. The question regarding the substrate specificity of the enzyme was therefore addressed. Recombinant 11-cis-RoDH was found capable of oxidizing and reducing 9-cis-, 11-cis- and 13-cis-isomers of retinol and retinaldehyde, respectively. Dodecyl-beta-1-maltoside used to solubilize the enzyme was found to affect the substrate specificity. This is the first report on a visual cycle enzyme also present in non-retinal ocular and non-ocular tissues. A possible role in addition to its role in the visual cycle is being discussed. |
