| First Author | Kreysing J | Year | 1994 |
| Journal | Genomics | Volume | 19 |
| Issue | 2 | Pages | 249-56 |
| PubMed ID | 7910580 | Mgi Jnum | J:16738 |
| Mgi Id | MGI:64802 | Doi | 10.1006/geno.1994.1055 |
| Citation | Kreysing J, et al. (1994) Structure of the mouse arylsulfatase A gene and cDNA. Genomics 19(2):249-56 |
| abstractText | The murine arylsulfatase A (ARSA) gene and cDNA have been cloned and sequenced. The gene is 3.8 kb long and contains eight exons. All intron/exon splice junctions conform to the GT/AG consensus sequence. The genomic structure is similar to that of the human gene. One major RNA species of 3.2 kb is transcribed. This RNA species has a 5' untranslated region of 638 nucleotides and terminates in a region around nucleotide 700 downstream of the termination codon. In addition, a rare mRNA species terminating at a polyadenylation signal 135 nucleotides downstream of the termination codon has been found. A larger transcript of 4 kb can be detected in liver. The size difference is due to initiation of transcription 5' of the cap site of the 3.2-kb mRNA species. The entire ARSA cDNA has been cloned by PCR from reverse-transcribed RNA. The coding sequence has 1518 nucleotides and predicts a protein of 506 amino acids. The nucleotide as well as the amino acid sequence is highly conserved among humans and mice. |
