| First Author | Trottier Y | Year | 1995 |
| Journal | Nat Genet | Volume | 10 |
| Issue | 1 | Pages | 104-10 |
| PubMed ID | 7647777 | Mgi Jnum | J:24940 |
| Mgi Id | MGI:72655 | Doi | 10.1038/ng0595-104 |
| Citation | Trottier Y, et al. (1995) Cellular localization of the Huntington's disease protein and discrimination of the normal and mutated form [see comments]. Nat Genet 10(1):104-10 |
| abstractText | Huntington's disease (HD) results from the expansion of a polyglutamine encoding CAG repeat in a gene of unknown function. The wide expression of this transcript does not correlate with the pattern of neuropathology in HD. To study the HD gene product (huntingtin), we have developed monoclonal antibodies raised against four different regions of the protein. On western blots, these monoclonals detect the approximately 350 kD huntingtin protein in various human cell lines and in neural and non-neural rodent tissues. In cell lines from HD patients, a doublet protein is detected corresponding to the mutated and normal huntingtin. Immunohistochemical studies in the human brain using two of these antibodies detects the huntingtin in perikarya of some neurons, neuropiles, varicosities and as punctate staining likely to be nerve endings. |
