| First Author | López-Alãnón DM | Year | 1997 |
| Journal | DNA Cell Biol | Volume | 16 |
| Issue | 5 | Pages | 639-44 |
| PubMed ID | 9174168 | Mgi Jnum | J:41139 |
| Mgi Id | MGI:892941 | Doi | 10.1089/dna.1997.16.639 |
| Citation | Lopez-Alanon DM, et al. (1997) H3.3A variant histone mRNA containing an alpha-globin insertion: modulated expression during mouse gametogenesis correlates with meiotic onset. DNA Cell Biol 16(5):639-44 |
| abstractText | Replacement-variant H3.3 histones have been isolated and sequenced in different eukaryotes, but no functional H3.3A gene has been characterized in the mouse so far. We have cloned an H3.3A cDNA from a mouse fetal ovary library, differentially screened with testis versus somatic cDNA probes. We showed this gene contains a region homologous to the reverse and complementary alpha-globin gene, We believe such a structure could have been generated by retroposition during the evolution of both globin and histone gene families. The sequence coding for H3.3A is 76.6% homologous to the mouse H3.3B gene at the nucleotide level and differs in only one amino acid at the protein level. The high degree of homology between these genes and the H3.3 variant histones from other eukaryotes reveals the conservation of these replication-independent class of histones throughout evolution, Analysis of gene expression reveals a developmental regulation concurrent with meiotic progression, with the highest level of transcript detection coincident with meiotic onset during both oogenesis and spermatogenesis. |
