| First Author | Abe TK | Year | 1997 |
| Journal | Biochem Biophys Res Commun | Volume | 233 |
| Issue | 2 | Pages | 295-9 |
| PubMed ID | 9144527 | Mgi Jnum | J:39843 |
| Mgi Id | MGI:87192 | Doi | 10.1006/bbrc.1997.6447 |
| Citation | Abe TK, et al. (1997) The presence of the 50-kDa subunit of dynactin complex in the nerve growth cone. Biochem Biophys Res Commun 233(2):295-9 |
| abstractText | Membrane proteins in growth cone-enriched and growth cone-non-enriched fractions prepared from neonatal mouse brain were separated by lectin-affinity and ion exchange chromatographies, 2D-PAGE, and SDS-PAGE. Partial amino acid sequences of the proteins concentrated in the growth cone-enriched fraction were determined. We found that one such protein, gmp23-48k, corresponds to the 50-kDa subunit (p50) of the dynactin complex. An antibody raised against gmp23-48k strongly reacted with growth cones of differentiated neuronal precursor cells. Immunoblot analyses revealed that gmp23-48k was present both in membrane and in soluble fractions of neonatal brain. However, the amount of gmp23-48k in the membrane fraction greatly decreased in adult brain. These results suggest a special role of membrane-associated gmp23-48k/p50 in synapse formation during brain development. |
