| First Author | Pandey A | Year | 2000 |
| Journal | Nat Immunol | Volume | 1 |
| Issue | 1 | Pages | 59-64 |
| PubMed ID | 10881176 | Mgi Jnum | J:63284 |
| Mgi Id | MGI:1860714 | Doi | 10.1038/76923 |
| Citation | Pandey A, et al. (2000) Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nat Immunol 1(1):59-64 |
| abstractText | Signaling by type I cytokines involves the formation of receptor homodimers, heterodimers or higher order receptor oligomers. Here we report the cloning of a type I cytokine receptor subunit that is most closely related to the common cytokine receptor gamma chain (gamma c). Binding and crosslinking experiments demonstrate that this protein is the receptor for a recently described interleukin 7 (IL-7)-like factor, thymic stromal lymphopoietin (TSLP). Binding of TSLP to the thymic stromal lymphopoietin receptor (TSLPR) is increased markedly in the presence of the IL-7 receptor alpha chain (IL-7R alpha). IL-7R alpha-expressing but not parental 32D cells proliferate in the presence of exogenous TSLP. Moreover, a combination of IL-7R alpha and TSLPR is required for TSLP-dependent activation of a STAT5-dependent reporter construct. Thus it is shown that IL-7R alpha is a component of both the IL-7 and TSLP receptors, which helps to explain why deletion of the gene that encodes IL-7R alpha affects the lymphoid system more severely than deletion of the gene encoding IL-7 does. Cloning of TSLPR should facilitate an understanding of TSLP function and its signaling mechanism. |
