| First Author | Hitsumoto Y | Year | 1992 |
| Journal | Biochem Biophys Res Commun | Volume | 187 |
| Issue | 2 | Pages | 773-7 |
| PubMed ID | 1530634 | Mgi Jnum | J:125146 |
| Mgi Id | MGI:3757625 | Doi | 10.1016/0006-291x(92)91262-o |
| Citation | Hitsumoto Y, et al. (1992) Purification of the murine heat-stable antigen from erythrocytes. Biochem Biophys Res Commun 187(2):773-7 |
| abstractText | The rat anti-mouse erythrocyte (MRBC) monoclonal antibody (mAb), R13, has been developed. The MRBC membrane protein recognized by R13 (R13-Ag) can be purified by loading the butanol-extracted MRBC membrane solution on a R13-conjugated Cellulofine column in the presence of 0.1% CHAPS followed by elution with 1% CHAPS. The amino acid sequence of the affinity-purified R13-Ag corresponded to that predicted from the cDNA for the murine heat-stable antigen. It was revealed that the actual heat-stable antigen was composed of 27 amino acids. |
