| First Author | Wang J | Year | 2012 |
| Journal | PLoS One | Volume | 7 |
| Issue | 1 | Pages | e30209 |
| PubMed ID | 22276159 | Mgi Jnum | J:184300 |
| Mgi Id | MGI:5320696 | Doi | 10.1371/journal.pone.0030209 |
| Citation | Wang J, et al. (2012) The N-terminus of FILIA forms an atypical KH domain with a unique extension involved in interaction with RNA. PLoS One 7(1):e30209 |
| abstractText | FILIA is a member of the recently identified oocyte/embryo expressed gene family in eutherian mammals, which is characterized by containing an N-terminal atypical KH domain. Here we report the structure of the N-terminal fragment of FILIA (FILIA-N), which represents the first reported three-dimensional structure of a KH domain in the oocyte/embryo expressed gene family of proteins. The structure of FILIA-N revealed a unique N-terminal extension beyond the canonical KH region, which plays important roles in interaction with RNA. By co-incubation with the lysates of mice ovaries, FILIA and FILIA-N could sequester specific RNA components, supporting the critical roles of FILIA in regulation of RNA transcripts during mouse oogenesis and early embryogenesis. |
