| First Author | Liu CH | Year | 1997 |
| Journal | Genomics | Volume | 43 |
| Issue | 1 | Pages | 15-24 |
| PubMed ID | 9226368 | Mgi Jnum | J:41657 |
| Mgi Id | MGI:894187 | Doi | 10.1006/geno.1997.4759 |
| Citation | Liu CH, et al. (1997) The mouse gene for the inducible G-protein-coupled receptor edg-1. Genomics 43(1):15-24 |
| abstractText | Edg-1, an immediate-early gene induced during the in vitro differentiation of human endothelial cells, encodes a G-protein-coupled receptor (GPR) that signals via the G(i)/mitogen-activated protein kinase (MAP kinase) pathway (Lee, M.-J., Evans, M., and Hla, T. (1996) J. Biol. Chem. 271, 11272-11279). It is a prototypical member of the subfamily of orphan receptors that are expressed in the cardiovascular and nervous systems. In this report, the mouse edg-1 gene was cloned and sequenced, and its expression patterns were defined. The edg-1 transcript was expressed in a wide variety of adult tissues including the brain, lung, liver, heart, and spleen. However, during embryogenesis, the edg-1 mRNA was induced late in development (after Embryonic Day 15.5) at centers of ossification. As a first step toward understanding the molecular basis of tissue-specific and inducible expression, the mouse edg-1 gene and its promoter were characterized. The mouse edg-1 gene is composed of two exons and is 4.9 kb in length. The second exon is large and contains the entire coding region and the 3'-untranslated region. The edg-1 promoter is TATA-less and contains GC-rich elements, and transcription initiation occurs from a single start site. The 5'-flanking region of the promoter contains several enhancer elements. However, the activity of the 5'-flanking region was suppressed by the repressor activity within the first intron. These data provide the basis for the further characterization of the regulation of the orphan GPR edg-1. |
