| First Author | Huo L | Year | 1996 |
| Journal | J Immunol | Volume | 157 |
| Issue | 9 | Pages | 3812-8 |
| PubMed ID | 8892610 | Mgi Jnum | J:36289 |
| Mgi Id | MGI:83754 | Doi | 10.4049/jimmunol.157.9.3812 |
| Citation | Huo L, et al. (1996) Isolation and characterization of murine fra-1: induction mediated by CD40 and surface Ig is protein kinase C dependent. J Immunol 157(9):3812-8 |
| abstractText | The murine fra-1 gene, encoding Fos-related Ag 1, was isolated from a splenic cDNA library and sequenced, Murine fra-1 was highly homologous to rat and human fra-1. Oligonucleotide primers based on the murine sequence were used to construct a quantitative reverse transcription-PCR assay for gene expression, B lymphocyte stimulation via both CD40 and surface Ig (sIg) receptors substantially induced fra-1 expression, and for bath receptors, induction was protein kinase C (PKC) dependent, This contrasts with induction of c-fos by both CD40 and sIg, which is PKC independent and indicates that CD40 is capable of signaling through PKC or a closely related kinase. Induction of fra-1 following engagement of CD40 did not require protein synthesis, suggesting that the PKC- dependent linkage between CD40 and fra-1 is direct. CD40- mediated fra-1 induction did require tyrosine kinase activity, These results demonstrate that CD40, like sIg, may employ PKC in producing select outcomes, that individual B cell receptors may signal downstream events via both PKC-dependent and PKC-independent pathways, and that multiple signal transduction pathways may be used to activate the expression of closely related genes. |
