| First Author | Schöler HR | Year | 1990 |
| Journal | Nature | Volume | 344 |
| Issue | 6265 | Pages | 435-9 |
| PubMed ID | 1690859 | Mgi Jnum | J:27203 |
| Mgi Id | MGI:74622 | Doi | 10.1038/344435a0 |
| Citation | Scholer HR, et al. (1990) New type of POU domain in germ line-specific protein Oct-4. Nature 344(6265):435-9 |
| abstractText | Members of a family of murine octamer-binding proteins interact specifically with the octamer motif, a transcription regulatory element found in the promoter and enhancer regions of many genes. Oct-4 is a maternally expressed protein that is also present in the pre-implantation mouse embryo. Although many regulatory proteins are expressed in post-implantation embryos, transcription factors regulating pre-implantation processes have remained elusive. The Oct-4 gene is therefore a prime candidate for an early developmental control gene. Here we report the complementary DNA cloning of the mouse Oct-4 gene, and the characterization of the encoded protein(s) by sequential in vitro transcription, translation, DNA-binding and protease-clipping analysis. Deletion analysis shows that the DNA-binding activity is mediated by a POU domain encoded in an open reading frame corresponding to a 324-amino-acid protein. Sequence comparison with known POU domains reveals that Oct-4 is a novel member of the POU-family. |
