| First Author | Okamoto K | Year | 1990 |
| Journal | Cell | Volume | 60 |
| Issue | 3 | Pages | 461-72 |
| PubMed ID | 1967980 | Mgi Jnum | J:10293 |
| Mgi Id | MGI:58746 | Doi | 10.1016/0092-8674(90)90597-8 |
| Citation | Okamoto K, et al. (1990) A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell 60(3):461-72 |
| abstractText | We have identified a novel octamer binding factor (Oct-3) in P19 embryonal carcinoma cells. Oct-3, which recognizes the typical octamer motif (ATTTGCAT) as well as the AT-rich sequence TTAAAATTCA, is present in P19 stem cells but disappears when the cells are induced to differentiate by retinoic acid (RA). Cloned cDNA corresponding to Oct-3 encodes a protein of 377 amino acids. Oct-3 has a conserved POU domain, but the remaining part is distinct from other POU domain-containing proteins such as Oct-1 and Oct-2. mRNA of 1.5 kb coding for Oct-3 is abundant in P19 stem cells but is dramatically repressed during RA-induced differentiation. Repression of the 1.5 kb mRNA is rapid and specific to RA. In mouse, oct-3 mRNA is undetectable in all the adult organs examined. The N-terminal proline-rich region of Oct-3, when fused to the DNA binding domain of c-Jun, functions as a transcriptional activating domain. We suggest that Oct-3 is a novel octamer binding transcription factor that is developmentally regulated during mouse embryogenesis. |
