| First Author | Blesch A | Year | 1994 |
| Journal | Cancer Res | Volume | 54 |
| Issue | 21 | Pages | 5695-701 |
| PubMed ID | 7923218 | Mgi Jnum | J:43389 |
| Mgi Id | MGI:1097587 | Citation | Blesch A, et al. (1994) Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA. Cancer Res 54(21):5695-701 |
| abstractText | Growth and progression of malignant melanoma cells is influenced by a complex network of growth-stimulating and -inhibiting factors produced by both the tumor cells and the local environment. Here we report the purification and molecular cloning of a novel growth regulating protein, designated melanoma inhibitory activity (MIA) and provide a preliminary functional characterization. MIA is translated as a 131-amino acid precursor and processed into a mature 107-amino acid protein after cleavage of a putative secretion signal. A murine complementary DNA was isolated that encoded a MIA-protein with 88% amino acid identity. MIA is secreted into the culture supernatant by several malignant melanoma cell lines as an M(r) 11,000 autocrine growth factor and acts as a potent tumor cell growth inhibitor for malignant melanoma cells and some other neuroectodermal tumors, including gliomas. MIA has no homology to any other known protein and, therefore, represents a novel type of growth-regulatory factor. Furthermore, we describe a molecular approach to express functionally active MIA in Escherichia coli, which might be attractive as a future antitumor therapeutical substance. |
