| First Author | Di Matteo G | Year | 1998 |
| Journal | J Biol Chem | Volume | 273 |
| Issue | 1 | Pages | 495-505 |
| PubMed ID | 9417108 | Mgi Jnum | J:45037 |
| Mgi Id | MGI:1101651 | Doi | 10.1074/jbc.273.1.495 |
| Citation | Di Matteo G, et al. (1998) Interactions with single-stranded and double-stranded DNA-binding factors and alternative promoter conformation upon transcriptional activation of the Htf9-a/RanBP1 and Htf9-c genes. J Biol Chem 273(1):495-505 |
| abstractText | The murine Htf9-a/RanBP1 and Htf9-c genes are divergently transcribed from a shared TATA-less promoter. Transcription of both genes is initiated on complementary DNA strands and is controlled by cell cycle-dependent mechanisms, The bidirectional promoter harbors a genomic footprint flanking the major transcription start site of both genes, Transient promoter assays showed that the footprinted element is important for transcription of both genes. Protein-binding experiments and antibody assays indicated that members of the retinoid X receptor family interact with the double-stranded site, In addition, distinct factors interact with single DNA strands of the element, Double-stranded binding factors were highly expressed in liver cells, in which neither gene is transcribed, while single-stranded binding proteins were abundant in cycling cells, in which transcription of both genes is efficient, In vivo S1 analysis of the promoter depicted an S1-sensitive organization in cells in which transcription of both genes is active; SI sensitivity was not detected in conditions of transcriptional repression, Thus, the same element is a target for either retinoid X receptor factors, or for single-stranded binding proteins, and form distinct complexes in different cellular conditions depending on the DNA conformation in the binding site. |
