| First Author | Sone Y | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 330 |
| Issue | 3 | Pages | 690-4 |
| PubMed ID | 15809052 | Mgi Jnum | J:97438 |
| Mgi Id | MGI:3575455 | Doi | 10.1016/j.bbrc.2005.03.032 |
| Citation | Sone Y, et al. (2005) Nuclear translocation of phospholipase C-zeta, an egg-activating factor, during early embryonic development. Biochem Biophys Res Commun 330(3):690-694 |
| abstractText | Phospholipase C-zeta (PLCzeta), a strong candidate of the egg-activating sperm factor, causes intracellular Ca(2+) oscillations and egg activation, and is subsequently accumulated into the pronucleus (PN), when expressed in mouse eggs by injection of RNA encoding PLCzeta. Changes in the localization of expressed PLCzeta were investigated by tagging with a fluorescent protein. PLCzeta began to translocate into the PN formed at 5-6h after RNA injection and increased there. Observation in the same embryo revealed that PLCzeta in the PN dispersed to the cytoplasm upon nuclear envelope breakdown and translocated again into the nucleus after cleavage. The dynamics was found in the second mitosis as well. When RNA was injected into fertilization-originated 1-cell embryos or blastomere(s) of 2-8-cell embryos, the nuclear localization of expressed PLCzeta was recognized in every embryo up to blastocyst. Thus, PLCzeta exhibited alternative cytoplasm/nucleus localization during development. This supports the view that the sperm factor could control cell cycle-dependent generation of Ca(2+) oscillations in early embryogenesis. |
