| First Author | Tashiro K | Year | 1993 |
| Journal | Science | Volume | 261 |
| Issue | 5121 | Pages | 600-3 |
| PubMed ID | 8342023 | Mgi Jnum | J:17644 |
| Mgi Id | MGI:65677 | Doi | 10.1126/science.8342023 |
| Citation | Tashiro K, et al. (1993) Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins. Science 261(5121):600-3 |
| abstractText | A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5' portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1 alpha (SDF-1 alpha) and SDF-1 beta, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned. |
