| First Author | Urano T | Year | 1992 |
| Journal | FEBS Lett | Volume | 309 |
| Issue | 3 | Pages | 358-62 |
| PubMed ID | 1325378 | Mgi Jnum | J:2262 |
| Mgi Id | MGI:50786 | Doi | 10.1016/0014-5793(92)80807-s |
| Citation | Urano T, et al. (1992) Molecular cloning and functional expression of the second mouse nm23/NDP kinase gene, nm23-M2. FEBS Lett 309(3):358-62 |
| abstractText | A new murine cDNA of nm23/NDP kinase was isolated. A RT-PCR product was obtained from the normal mouse liver mRNA with primers designed for the human nm23-H2 gene. The product was used as a probe to screen a cDNA library from the murine melanoma cell line, B16, and two clones containing the entire open reading frame were obtained. It was predicted that the DNA sequence encoded 152 amino acids which was 98% identical to the nm23-H2 protein. The entire nm23-M1 and -M2 gene-coding regions were translated as fusion proteins with a glutathione S-transferase. These fusion proteins displayed NDP kinase activities. |
