| First Author | Willison KR | Year | 1986 |
| Journal | Cell | Volume | 44 |
| Issue | 5 | Pages | 727-38 |
| PubMed ID | 3753900 | Mgi Jnum | J:8190 |
| Mgi Id | MGI:56659 | Doi | 10.1016/0092-8674(86)90839-1 |
| Citation | Willison KR, et al. (1986) Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1. Cell 44(5):727-38 |
| abstractText | The mouse t haplotypes show defects in spermatogenesis attributed to multiple loci on chromosome 17. We have cloned the gene for an abundant testicular germ cell protein, t complex polypeptide 1, which has a variant form in t haplotypes, TCP-1A. A cDNA clone, pB1.4, which hybridizes to a 19S mRNA that is abundant in haploid cells during mouse spermatogenesis, derives from the 3' end of the mRNA encoding TCP-1B. The Tcp-1 gene appears to be a member of a novel gene family and shows multiple changes between the predicted amino acid sequences of TCP-1B and TCP-1A. An additional Taq1 site is created by a T to C transition in the predicted open reading frame of the Tcp-1a gene. The resultant RFLP has allowed typing of the Tcp-1 gene cluster in 54 complete and partial t haplotype chromosomes. DNA sequence comparison of the Tcp-1 genes suggests that the t haplotype chromosome arose within the genus Mus more than one million years ago. |
