| First Author | Yamagata T | Year | 1997 |
| Journal | Genomics | Volume | 45 |
| Issue | 3 | Pages | 535-40 |
| PubMed ID | 9367678 | Mgi Jnum | J:44254 |
| Mgi Id | MGI:1099629 | Doi | 10.1006/geno.1997.4966 |
| Citation | Yamagata T, et al. (1997) Genome organization of human 48-kDa oligosaccharyltransferase (DDOST). Genomics 45(3):535-40 |
| abstractText | The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase; EC 2. 4.1.119) (DDOST) catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. We isolated mouse and human DDOST cDNAs from retinoic acid-treated mouse P19 EC cells and human NT-2 cells, respectively. DDOST mRNA is expressed intensely in heart and pancreas, but at lower levels in brain. Here we show that the human DDOST 48-kDa subunit gene (HGMW-approved symbol DDOST) is organized into 11 exons expanding about 9 kb. This DDOST subunit gene is localized on chromosome 1p36.1 by fluorescence in situ hybridization analysis. Copyright 1997 Academic Press. |
