| First Author | Kauppi L | Year | 2011 |
| Journal | Science | Volume | 331 |
| Issue | 6019 | Pages | 916-20 |
| PubMed ID | 21330546 | Mgi Jnum | J:169057 |
| Mgi Id | MGI:4939810 | Doi | 10.1126/science.1195774 |
| Citation | Kauppi L, et al. (2011) Distinct properties of the XY pseudoautosomal region crucial for male meiosis. Science 331(6019):916-20 |
| abstractText | Meiosis requires that each chromosome find its homologous partner and undergo at least one crossover. X-Y chromosome segregation hinges on efficient crossing-over in a very small region of homology, the pseudoautosomal region (PAR). We find that mouse PAR DNA occupies unusually long chromosome axes, potentially as shorter chromatin loops, predicted to promote double-strand break (DSB) formation. Most PARs show delayed appearance of RAD51/DMC1 foci, which mark DSB ends, and all PARs undergo delayed DSB-mediated homologous pairing. Analysis of Spo11beta isoform-specific transgenic mice revealed that late RAD51/DMC1 foci in the PAR are genetically distinct from both early PAR foci and global foci and that late PAR foci promote efficient X-Y pairing, recombination, and male fertility. Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination. |
