| First Author | Sagné C | Year | 1997 |
| Journal | FEBS Lett | Volume | 417 |
| Issue | 2 | Pages | 177-83 |
| PubMed ID | 9395291 | Mgi Jnum | J:44006 |
| Mgi Id | MGI:1099257 | Doi | 10.1016/s0014-5793(97)01279-9 |
| Citation | Sagne C, et al. (1997) Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases. FEBS Lett 417(2):177-83 |
| abstractText | The unc-47 locus of Caenorhabditis elegans has been suggested to encode a synaptic vesicle GABA transporter. Here we used hydropathy plot analysis to identify a candidate vesicular GABA transporter in genomic sequences derived from a region of the physical map comprising unc-47. A mouse homologue was identified and cloned from EST database information. In situ hybridization in rat brain revealed codistribution with both GABAergic and glycinergic neuronal markers. Moreover, expression in COS-7 and PC12 cells induced an intracellular, glycine-sensitive GABA uptake activity. These observations, consistent with previous data on GABA and glycine uptake by synaptic vesicles, demonstrate that the mouse clone encodes a vesicular inhibitory amino acid transporter. |
