| First Author | Ugawa S | Year | 2001 |
| Journal | Am J Physiol Gastrointest Liver Physiol | Volume | 281 |
| Issue | 2 | Pages | G365-70 |
| PubMed ID | 11447016 | Mgi Jnum | J:326288 |
| Mgi Id | MGI:7310204 | Doi | 10.1152/ajpgi.2001.281.2.G365 |
| Citation | Ugawa S, et al. (2001) Characterization of a mouse colonic system B(0+) amino acid transporter related to amino acid absorption in colon. Am J Physiol Gastrointest Liver Physiol 281(2):G365-70 |
| abstractText | Previous experiments have shown that an amino acid transport system B(0+) transporter in cultured colonic epithelial cells mediates amino acid absorption. Here we describe the cloning and functional characterization of a system B(0+) transporter selectively expressed in the colon. Using the combination of an expressed sequence tag database search and RT-PCR approaches, we cloned a mouse colonic amino acid transporter, designated mCATB(0+). Northern blot analysis revealed that mCATB(0+) was selectively expressed in the large intestine. In situ hybridization showed the mCATB(0+) mRNA to be localized in absorptive epithelial cells. When expressed in Xenopus oocytes, mCATB(0+) exhibited a Na(+)-dependent stereoselective uptake and a broad specificity for neutral and cationic amino acids, which is characteristic of amino acid transport system B(0+). In vivo [(3)H]glycine uptake assay demonstrated that a system B(0+)-like transporter protein was expressed on the apical surface of the colonic absorptive cells. Our data suggest that a mouse colonic amino acid transporter mCATB(0+) may absorb amino acids from the intestinal contents in the colon. |
