| First Author | Hwang ES | Year | 2002 |
| Journal | J Immunol | Volume | 169 |
| Issue | 1 | Pages | 248-53 |
| PubMed ID | 12077251 | Mgi Jnum | J:132921 |
| Mgi Id | MGI:3777198 | Doi | 10.4049/jimmunol.169.1.248 |
| Citation | Hwang ES, et al. (2002) Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1. J Immunol 169(1):248-53 |
| abstractText | GATA-3 is a T cell-specific transcription factor and is essential for the development of the T cell lineage. The transcriptional regulation of GATA-3, however, remains elusive. In this study, we report the identification of a regulatory region located within the first intron of the murine GATA-3 gene. The intronic regulatory region contains both a positive and a negative cis-acting element but, as a whole, serves as a potent T cell-specific enhancer and is essential for the promoter activity in vitro. By using yeast one-hybrid screening, we discovered that fetal liver zinc finger protein 1 (Fliz1) could bind specifically to the negative cis-acting element, the sequence of which is conserved between the mouse and human GATA-3 genes. More importantly, overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. Our data suggest that the expression of GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion. |
