| First Author | Dunkelberg JC | Year | 2001 |
| Journal | DNA Cell Biol | Volume | 20 |
| Issue | 7 | Pages | 403-12 |
| PubMed ID | 11506704 | Mgi Jnum | J:70705 |
| Mgi Id | MGI:2138022 | Doi | 10.1089/104454901750361460 |
| Citation | Dunkelberg JC, et al. (2001) Lz-fyve: a novel developmental stage-specific leucine zipper, fyve-finger protein. DNA Cell Biol 20(7):403-12 |
| abstractText | We describe the molecular cloning and characterization of LZ-FYVE, a novel embryonic factor that possesses two leucine zipper motifs and a FYVE-finger domain. A partial clone of LZ-FYVE, encoding a functional leucine zipper domain, was initially isolated from a mouse embryo cDNA library by virtue of its interaction in the yeast two-hybrid system with the transcription factor ATF-2. The LZ-FYVE protein demonstrated mouse embryo-specific expression by Northern blot analysis and was detected as a nuclear protein at very restricted periods (12-14 days post-coitus) in specific tissues during embryogenesis. In particular, LZ-FYVE protein was notable in embryonic lung, cartilage, and otic capsule. Structural analysis of the deduced, full-length LZ-FYVE amino acid sequence revealed two N-terminal leucine zipper domains as well as a C-terminal FYVE-finger domain. The FYVE-finger domains specifically recognized phosphatidylinositol 3-phosphate and have been previously described only in cytoplasmic proteins involved in endosomal membrane fusion, vesicular trafficking, or organelle-specific targeting. While LZ-FYVE may have endosomal functions in the cytoplasm, because LZ-FYVE is present in the nucleus at early stages of embryonic development, it is likely that LZ-FYVE has a nuclear function. |
