| First Author | Jacks CM | Year | 1988 |
| Journal | Nucleic Acids Res | Volume | 16 |
| Issue | 22 | Pages | 10751-64 |
| PubMed ID | 2462715 | Mgi Jnum | J:31499 |
| Mgi Id | MGI:79008 | Doi | 10.1093/nar/16.22.10751 |
| Citation | Jacks CM, et al. (1988) Analysis of potential expression of highly related members of the ribosomal protein L32 gene family. Nucleic Acids Res 16(22):10751-64 |
| abstractText | The processed gene L32', a member of the mouse gene family for ribosomal protein L32, could encode a 135 amino acid protein nearly identical to L32. The 5'-flanking region of the gene contains CAAT and TATA sites at positions commonly found in expressed genes. The L32' gene lies within highly methylated, DNase I-insensitive chromatin of mouse L1210 cells. Although S1 nuclease digestion studies suggested that an L32' transcript might be produced, an oligonucleotide probe specific for L32' mRNA, and RNase digestion of a cRNA probe to L32', indicated fewer than 0.1 L32' transcripts/cell. These results demonstrate that extreme caution is required when measuring transcription from related genes. |
