| First Author | Li S | Year | 2007 |
| Journal | Dev Biol | Volume | 301 |
| Issue | 1 | Pages | 106-16 |
| PubMed ID | 16982049 | Mgi Jnum | J:116977 |
| Mgi Id | MGI:3695332 | Doi | 10.1016/j.ydbio.2006.07.048 |
| Citation | Li S, et al. (2007) GLP-1: a novel zinc finger protein required in somatic cells of the gonad for germ cell development. Dev Biol 301(1):106-16 |
| abstractText | Mouse gonadal development is regulated by a variety of transcription factors. Here we report the identification and characterization of a novel nuclear zinc finger protein called GATA like protein-1 (GLP-1), which is expressed at high levels in the somatic cells of the developing gonads, including Leydig cells in the testes and granulosa cells in the ovaries. Biochemical analysis of GLP-1 shows that it acts as a transcriptional repressor of GATA factor function. To determine the necessity of GLP-1 in gonadal development, a null allele in mice was generated by replacing all of the coding exons with the bacterial lacZ gene. GLP-1(lacZ) null mice are viable with no detectable defects in visceral organ development; however, both males and females are completely infertile. Loss of GLP-1 leads to defective sperm development in males with a marked reduction in mature spermatids observed as early as postnatal week 1. In females, loss of GLP-1 leads to a severe block in germ cell development as early as E17.5. Together, these data identify GLP-1 as a critical nuclear repressor in somatic cells of the gonad that is required for germ cell development, and highlight the importance of somatic-germ cell interactions in the regulation of this critical process. |
