| First Author | Ratel D | Year | 2006 |
| Journal | FEBS Lett | Volume | 580 |
| Issue | 13 | Pages | 3179-84 |
| PubMed ID | 16684535 | Mgi Jnum | J:109076 |
| Mgi Id | MGI:3625673 | Doi | 10.1016/j.febslet.2006.04.074 |
| Citation | Ratel D, et al. (2006) Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase. FEBS Lett 580(13):3179-84 |
| abstractText | Three methylated bases, 5-methylcytosine, N4-methylcytosine and N6-methyladenine (m6A), can be found in DNA. However, to date, only 5-methylcytosine has been detected in mammalian genomes. To reinvestigate the presence of m6A in mammalian DNA, we used a highly sensitive method capable of detecting one N6-methyldeoxyadenosine per million nucleosides. Our results suggest that the total mouse genome contains, if any, less than 10(3) m6A. Experiments were next performed on PRED28, a putative mammalian N6-DNA methyltransferase. The murine PRED28 encodes two alternatively spliced RNA. However, although recombinant PRED28 proteins are found in the nucleus, no evidence for an adenine-methyltransferase activity was detected. |
