| First Author | Takenaka Y | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 6 | Pages | e65583 |
| PubMed ID | 23840343 | Mgi Jnum | J:203725 |
| Mgi Id | MGI:5528597 | Doi | 10.1371/journal.pone.0065583 |
| Citation | Takenaka Y, et al. (2013) A Novel Splicing Variant of Peroxisome Proliferator-Activated Receptor-gamma () Cooperatively Regulates Adipocyte Differentiation with. PLoS One 8(6):e65583 |
| abstractText | Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors that regulate expression of a number of genes associated with the cellular differentiation and development. Here, we show the abundant and ubiquitous expression of a newly identified splicing variant of mouse Ppargamma (Ppargamma1sv) that encodes PPARgamma1 protein, and its importance in adipogenesis. The novel splicing variant has a unique 5'-UTR sequence, relative to those of Ppargamma1 and Ppargamma2 mRNAs, indicating the presence of a novel transcriptional initiation site and promoter for Ppargamma expression. Ppargamma1sv was highly expressed in the white and brown adipose tissues at levels comparable to Ppargamma2. Ppargamma1sv was synergistically up-regulated with Ppargamma2 during adipocyte differentiation of 3T3-L1 cells and mouse primary cultured preadipocytes. Inhibition of Ppargamma1sv by specific siRNAs completely abolished the induced adipogenesis in 3T3-L1 cells. C/EBPbeta and C/EBPdelta activated both the Ppargamma1sv and Ppargamma2 promoters in 3T3-L1 preadipocytes. These findings suggest that Ppargamma1sv and Ppargamma2 synergistically regulate the early stage of the adipocyte differentiation. |
