| First Author | Göttgens B | Year | 2000 |
| Journal | Nat Biotechnol | Volume | 18 |
| Issue | 2 | Pages | 181-6 |
| PubMed ID | 10657125 | Mgi Jnum | J:60317 |
| Mgi Id | MGI:1353160 | Doi | 10.1038/72635 |
| Citation | Gottgens B, et al. (2000) Analysis of vertebrate SCL loci identifies conserved enhancers [see comments]. Nat Biotechnol 18(2):181-6 |
| abstractText | The SCL gene encodes a highly conserved bHLH transcription factor with a pivotal role in hemopoiesis and vasculogenesis. We have sequenced and analyzed 320 kb of genomic DNA composing the SCL loci from human, mouse, and chicken. Long-range sequence comparisons demonstrated multiple peaks of human/mouse homology, a subset of which corresponded precisely with known SCL enhancers. Comparisons between mammalian and chicken sequences identified some, but not all, SCL enhancers. Moreover, one peak of human/mouse homology (+23 region), which did not correspond to a known enhancer, showed significant homology to an analogous region of the chicken SCL locus. A transgenic Xenopus reporter assay was established and demonstrated that the +23 region contained a new neural enhancer. This combination of long-range comparative sequence analysis with a high-throughput transgenic bioassay provides a powerful strategy for identifying and characterizing developmentally important enhancers. |
