| First Author | Liang H | Year | 2017 |
| Journal | Nat Commun | Volume | 8 |
| Pages | 14771 | PubMed ID | 28332494 |
| Mgi Jnum | J:244283 | Mgi Id | MGI:5913062 |
| Doi | 10.1038/ncomms14771 | Citation | Liang H, et al. (2017) PTENbeta is an alternatively translated isoform of PTEN that regulates rDNA transcription. Nat Commun 8:14771 |
| abstractText | PTEN is a critical tumour suppressor that is frequently mutated in human cancer. We have previously identified a CUG initiated PTEN isoform designated PTENalpha, which functions in mitochondrial bioenergetics. Here we report the identification of another N-terminal extended PTEN isoform, designated PTENbeta. PTENbeta translation is initiated from an AUU codon upstream of and in-frame with the AUG initiation sequence for canonical PTEN. We show that the Kozak context and a downstream hairpin structure are critical for this alternative initiation. PTENbeta localizes predominantly in the nucleolus, and physically associates with and dephosphorylates nucleolin, which is a multifunctional nucleolar phosphoprotein. Disruption of PTENbeta alters rDNA transcription and promotes ribosomal biogenesis, and this effect can be reversed by re-introduction of PTENbeta. Our data show that PTENbeta regulates pre-rRNA synthesis and cellular proliferation. These results demonstrate the complexity of the PTEN protein family and the diversity of its functions. |
