| First Author | Sakano H | Year | 1981 |
| Journal | Nature | Volume | 290 |
| Issue | 5807 | Pages | 562-5 |
| PubMed ID | 6783961 | Mgi Jnum | J:6493 |
| Mgi Id | MGI:54969 | Doi | 10.1038/290562a0 |
| Citation | Sakano H, et al. (1981) Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes. Nature 290(5807):562-5 |
| abstractText | A putative diversity segment of immunoglobulin heavy-chain genes (D segments) has been identified 700 base pairs 5' to JH1 DNA on the germ-line genome of the mouse. This 10-base pair D segment is flanked by two sets of sequences related to (SEE FORMULAR IN TEXT) which are possible recognition sites for a recombinase. The spacer separating the heptamer and the nonamer is 12 base pairs long on both sides of the D segment. As the space separating the two signal sequences in VH DNAs and JH DNAs is 23 +/- 1 base pairs long, the two recombinations required for creation of a complete immunoglobulin VH gene, a VH--D joining and a D--JH joining, follow a 12/23-base pair spacer rule. Allelic exclusion is discussed with respect to D segments. |
