| First Author | Zhai J | Year | 2008 |
| Journal | Biochem Biophys Res Commun | Volume | 377 |
| Issue | 2 | Pages | 384-388 |
| PubMed ID | 18851954 | Mgi Jnum | J:143596 |
| Mgi Id | MGI:3828224 | Doi | 10.1016/j.bbrc.2008.09.147 |
| Citation | Zhai J, et al. (2008) Characterization of a novel isoform of murine interferon regulatory factor 3. Biochem Biophys Res Commun 377(2):384-8 |
| abstractText | Interferon (IFN) regulatory factors (IRFs) are a family of transcription mediators involved in the regulation of type I IFN (IFN-alpha/beta) transcription. Among the nine already identified IRFs, IRF-3 is a constitutively and ubiquitously expressed and plays a critical role in the transcriptional activation of type I IFN and IFN-stimulated genes including IFN-alpha1, IFN-beta and RANTES. In the present study, we report the identification of a novel alternatively spliced transcript of murine Irf-3 gene (mIrf-3) designated mIRF-3a. mIRF-3a is ubiquitously present in mouse tissues along with mIRF-3 and could be translocated into the nucleus in uninfected L929 cells. EMSA and reporter assay demonstrated that mIRF-3a binds to ISRE sequences in murine Ifn-beta promoter and represses Ifn-beta promoter activation induced by Newcastle disease virus infection. These results suggest that mIRF-3a may act as a modulator of mIRF-3 functions in vivo. |
