| First Author | Dunstan CA | Year | 1996 |
| Journal | J Biol Chem | Volume | 271 |
| Issue | 51 | Pages | 32770-6 |
| PubMed ID | 8955112 | Mgi Jnum | J:37299 |
| Mgi Id | MGI:84701 | Doi | 10.1074/jbc.271.51.32770 |
| Citation | Dunstan CAN, et al. (1996) Identification of two rat genes orthologous to the human interleukin-8 receptors. J Biol Chem 271(51):32770-6 |
| abstractText | The genes encoding two functional human interleukin-8 (IL-8) receptors have been identified by molecular cloning techniques and they are members of the rhodopsin G-protein coupled receptor (GCR) superfamily. We report the molecular cloning of two rat GCR genes (rat CXCR1-like and rat CXCR2) whose conceptualized amino acid sequences are approximately 70% identical to the human IL-8 A and B receptor subtypes. The murine GRO-like peptide, macrophage inflammatory peptide-2 (MIP-2), elevates intracellular calcium levels in HEK293 cells expressing the rat CXCR2 receptor. Southern blot analysis of restriction-digested rodent and human genomic DNAs indicate that rat CXCR1-like and CXCR2 are: 1) each single copy genes in the rat genome; 2) most closely related to the human IL-8 receptor genes; and 3) orthologous to two previously identified murine genes. CXCR2 mRNA is detected in adult rat lung, spleen, and neutrophils. CXCR1-like mRNA can be detected in adult rat lung, native rat macrophages, and a rat alveolar macrophage cell line (NR8383). These data identify the rat orthologs of the human IL-8 receptors, and describe cellular and tissue targets of rat C-X-C chemokine peptides. |
