| First Author | Gatfield J | Year | 2005 |
| Journal | Mol Biol Cell | Volume | 16 |
| Issue | 6 | Pages | 2786-98 |
| PubMed ID | 15800061 | Mgi Jnum | J:100752 |
| Mgi Id | MGI:3589500 | Doi | 10.1091/mbc.E05-01-0042 |
| Citation | Gatfield J, et al. (2005) Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules. Mol Biol Cell 16(6):2786-98 |
| abstractText | Coronin 1 is a member of the coronin protein family specifically expressed in leukocytes and accumulates at sites of rearrangements of the F-actin cytoskeleton. Here, we describe that coronin 1 molecules are coiled coil-mediated homotrimeric complexes, which associate with the plasma membrane and with the cytoskeleton via two distinct domains. Association with the cytoskeleton was mediated by trimerization of a stretch of positively charged residues within a linker region between the N-terminal, WD repeat-containing domain and the C-terminal coiled coil. In contrast, neither the coiled coil nor the positively charged residues within the linker domain were required for plasma membrane binding, suggesting that the N-terminal, WD repeat-containing domain mediates membrane interaction. The capacity of coronin 1 to link the leukocyte cytoskeleton to the plasma membrane may serve to integrate outside-inside signaling with modulation of the cytoskeleton. |
