| First Author | Mayeda A | Year | 1999 |
| Journal | EMBO J | Volume | 18 |
| Issue | 16 | Pages | 4560-70 |
| PubMed ID | 10449421 | Mgi Jnum | J:57040 |
| Mgi Id | MGI:1343609 | Doi | 10.1093/emboj/18.16.4560 |
| Citation | Mayeda A, et al. (1999) Purification and characterization of human RNPS1: a general activator of pre-mRNA splicing. EMBO J 18(16):4560-70 |
| abstractText | Biochemical purification of a pre-mRNA splicing activity from HeLa cells that stimulates distal alternative 3' splice sites in a concentration-dependent manner resulted in the identification of RNPS1, a novel general activator of pre-mRNA splicing. RNPS1 cDNAs, encoding a putative nucleic-acid-binding protein of unknown function, were previously identified in mouse and human. RNPS1 is conserved in metazoans and has an RNA-recognition motif preceded by an extensive serine-rich domain. Recombinant human RNPS1 expressed in baculovirus functionally synergizes with SR proteins and strongly activates splicing of both constitutively and alternatively spliced pre-mRNAs. We conclude that RNPS1 is not only a potential regulator of alternative splicing but may also play a more fundamental role as a general activator of pre-mRNA splicing. |
