| First Author | Stebel M | Year | 2000 |
| Journal | FEBS Lett | Volume | 481 |
| Issue | 2 | Pages | 152-8 |
| PubMed ID | 10996315 | Mgi Jnum | J:64788 |
| Mgi Id | MGI:1889982 | Doi | 10.1016/s0014-5793(00)02004-4 |
| Citation | Stebel M, et al. (2000) The growth suppressing gas1 product is a GPI-linked protein. FEBS Lett 481(2):152-8 |
| abstractText | Growth arrest specific (gas) 1 gene product is expressed in non-transformed fibroblasts in response to stimuli driving cells into Go phase. Gas1 has been demonstrated to inhibit cell proliferation when over-expressed in proliferating fibroblasts. This activity depends on a function of the p53 protein independent of its transactivating ability. To better define the pathway leading from Gas1, which is located on the plasma membrane, to p53, we have undertaken a detailed characterization of its topology. We demonstrate that the protein undergoes cotranslational modifications in the endoplasmic reticulum, consisting of signal peptide cleavage, N-linked glycosylation and glycosyl-phosphatidylinositol anchor addition. Immunoelectron microscopy shows that, in its mature form, Gas1 is randomly distributed over the outer leaflet of the plasma membrane and that upon antibody-induced clustering it relocalizes to caveolae. |
