| First Author | Kanatsu-Shinohara M | Year | 2022 |
| Journal | Cell Rep Med | Volume | 3 |
| Issue | 5 | Pages | 100606 |
| PubMed ID | 35584625 | Mgi Jnum | J:334695 |
| Mgi Id | MGI:7461973 | Doi | 10.1016/j.xcrm.2022.100606 |
| Citation | Kanatsu-Shinohara M, et al. (2022) Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. Cell Rep Med 3(5):100606 |
| abstractText | Oocytes and granulosa cells closely interact with each other during follicular development, and a lack of appropriate signaling between them results in infertility. Attempts to manipulate oocyte microenvironment have been impeded by the impermeability of the blood-follicle barrier (BFB). To establish a strategy for manipulating oogenesis, we use adeno-associated viruses (AAVs), which have a unique ability of transcytosis. Microinjecting of AAVs into the ovarian stroma penetrates the BFB and achieves long-term gene expression. Introduction of an AAV carrying the mouse Kitl gene restores oogenesis in congenitally infertile Kitl(Sl-t)/Kitl(Sl-t) mutant mouse ovaries, which lack Kitl expression but contain only primordial follicles. Healthy offspring without AAV integration are born by natural mating. Therefore, AAV-mediated gene delivery not only provides a means for studying oocyte-granulosa interactions through the manipulation of the oocyte microenvironment but could also be a powerful method to treat female infertility resulting from somatic cell defects. |
