| First Author | Choo HJ | Year | 2006 |
| Journal | Diabetologia | Volume | 49 |
| Issue | 4 | Pages | 784-91 |
| PubMed ID | 16501941 | Mgi Jnum | J:107887 |
| Mgi Id | MGI:3622487 | Doi | 10.1007/s00125-006-0170-2 |
| Citation | Choo HJ, et al. (2006) Mitochondria are impaired in the adipocytes of type 2 diabetic mice. Diabetologia 49(4):784-91 |
| abstractText | AIMS/HYPOTHESIS: The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes. MATERIALS AND METHODS: Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice. RESULTS: Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity. CONCLUSIONS/INTERPRETATION: Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes. |
