| First Author | Khang R | Year | 2015 |
| Journal | Neuroscience | Volume | 294 |
| Pages | 182-92 | PubMed ID | 25779963 |
| Mgi Jnum | J:222286 | Mgi Id | MGI:5644229 |
| Doi | 10.1016/j.neuroscience.2015.03.017 | Citation | Khang R, et al. (2015) Dysregulation of parkin in the substantia nigra of db/db and high-fat diet mice. Neuroscience 294:182-92 |
| abstractText | Parkinson's disease (PD) is characterized by selective loss of dopaminergic neurons in the substantia nigra (SN). Epidemiological evidence has suggested a link between type 2 diabetes and PD, although the mechanisms remain largely unknown. We applied LC-MS/MS-based pattern analysis to investigate altered proteomes in the SN of db/db mice (db-SN) and high-fat diet mice (HFD-SN), revealing that the level of mitochondrial proteins has changed in the SN of diabetic mice compared to that of control mice. Since mitochondrial proteins were robustly altered in db-SN and HFD-SN, we performed immunoblot analysis to monitor the level of parkin, PINK1 (phosphatase and tensin homolog-induced putative kinase 1) and DJ-1 that were directly involved in mitochondrial dynamics. As a result, PINK1 and DJ-1 level was unchanged, whereas a significant loss of parkin was found in db-SN and HFD-SN, leading to the accumulation of parkin-interacting substrate (PARIS) and the reduction of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha). Interestingly, these alterations were reversed by the administration of metformin, one of most frequently prescribed anti-hyperglycemic agents. The slight loss of dopaminergic neurons was found in chronic HFD-SN that was restored by metformin. Taken together, our data suggest that the dysregulation of Parkin-PARIS-PGC-1alpha pathway by metabolic malregulation may contribute to the pathogenesis of PD and metformin might exert a neuroprotective effect on PD via the restoration of parkin. |
