| First Author | Jung Y | Year | 2018 |
| Journal | FASEB J | Volume | 32 |
| Issue | 3 | Pages | 1388-1402 |
| PubMed ID | 29141998 | Mgi Jnum | J:270594 |
| Mgi Id | MGI:6277482 | Doi | 10.1096/fj.201700231RR |
| Citation | Jung Y, et al. (2018) Vanillic acid attenuates obesity via activation of the AMPK pathway and thermogenic factors in vivo and in vitro. FASEB J 32(3):1388-1402 |
| abstractText | Energy expenditure is a target gaining recent interest for obesity treatment. The antiobesity effect of vanillic acid (VA), a well-known flavoring agent, was investigated in vivo and in vitro. High-fat diet (HFD)-induced obese mice and genetically obese db/db mice showed significantly decreased body weights after VA administration. Two major adipogenic markers, peroxisome proliferator activated receptor gamma (PPARgamma) and CCAAT/enhancer-binding protein alpha (C/EBPalpha), were reduced while the key factor of energy metabolism, AMPKalpha, was increased in the white adipose tissue and liver tissue of VA-treated mice. Furthermore, VA inhibited lipid accumulation and reduced hepatotoxic/inflammatory markers in liver tissues of mice and HepG2 hepatocytes. VA treatment also decreased differentiation of 3T3-L1 adipocytes by regulating adipogenic factors including PPARgamma and C/EBPalpha. AMPKalpha small interfering RNA was used to examine whether AMPK was associated with the actions of VA. In AMPKalpha-nulled 3T3-L1 cells, the inhibitory action of VA on PPARgamma and C/EBPalpha was attenuated. Furthermore, in brown adipose tissues of mice and primary cultured brown adipocytes, VA increased mitochondria- and thermogenesis-related factors such as uncoupling protein 1 and PPARgamma-coactivator 1-alpha. Taken together, our results suggest that VA has potential as an AMPKalpha- and thermogenesis-activating antiobesity agent.-Jung, Y., Park, J., Kim, H.-L., Sim, J.-E., Youn, D.-H., Kang, J., Lim, S., Jeong, M.-Y., Yang, W. M., Lee, S.-G., Ahn, K. S., Um, J.-Y. Vanillic acid attenuates obesity via activation of the AMPK pathway and thermogenic factors in vivo and in vitro. |
