| First Author | Scoville DW | Year | 2020 |
| Journal | J Mol Endocrinol | Volume | 65 |
| Issue | 3 | Pages | 59-67 |
| PubMed ID | 32668405 | Mgi Jnum | J:345638 |
| Mgi Id | MGI:7610102 | Doi | 10.1530/JME-20-0082 |
| Citation | Scoville DW, et al. (2020) Identification of a novel lncRNA (G3R1) regulated by GLIS3 in pancreatic beta-cells. J Mol Endocrinol 65(3):59-67 |
| abstractText | Recent advances in high throughput RNA sequencing have revealed that, in addition to messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs) play an important role in the regulation of many cell functions and of organ development. While a number of lncRNAs have been identified in pancreatic islets, their function remains largely undetermined. Here, we identify a novel long ncRNA regulated by the transcription factor GLIS3, which we refer to as GLIS3 regulated 1 (G3R1). This lncRNA was identified for its significant loss of expression in GLIS3 knockout mouse pancreatic islets. G3R1 appears to be specifically expressed in mouse pancreatic beta-cells and in a beta-cell line (betaTC-6). ChIP-seq analysis indicated that GLIS3 and other islet-enriched transcription factors bind near the G3R1 gene, suggesting they directly regulate G3R1 transcription. Similarly, an apparent human homolog of G3R1 displays a similar expression pattern, with additional expression seen in human brain. In order to determine the function of G3R1 in mouse pancreatic beta-cells, we utilized CRISPR to develop a knockout mouse where ~80% of G3R1 sequence is deleted. Phenotypic analysis of these mice did not reveal any impairment in beta-cell function or glucose regulation, indicating the complexity underlying the study of lncRNA function. |
