| First Author | Sinclair A | Year | 1991 |
| Journal | Dev Neurosci | Volume | 13 |
| Issue | 3 | Pages | 138-42 |
| PubMed ID | 1721567 | Mgi Jnum | J:2529 |
| Mgi Id | MGI:51051 | Doi | 10.1159/000112151 |
| Citation | Sinclair A, et al. (1991) Shiverer jimpy double mutant mice. V. Correlation of genotype and myelin proteins. Dev Neurosci 13(3):138-42 |
| abstractText | We have reexamined the levels of myelin basic protein (MBP) and proteolipid protein (PLP) in the brains of mice bred to carry both the shi/shi and jp/Y hypomyelination defects. The genotype of each putative double mutant was confirmed by direct DNA analysis: shi/shi by Southern blot analysis, and jp/Y by restriction enzyme analysis of polymerase chain reaction-amplified fragments. MBP and PLP levels were assessed by immunoblotting. All putative double mutants were found to be shi/shi. However, examination of the PLP locus revealed both jp and wild-type genotypes, the latter produced by an expected crossover. Animals proven to be shi/shi*jp/Y had no detectable MBP or PLP; those proven to be shi/shi*+/Y (the crossover) had no MBP but had PLP. These results differ from an earlier report of both MBP and PLP in the brains of presumed shi*jp animals. |
