| First Author | Karthigasan J | Year | 1996 |
| Journal | J Neurochem | Volume | 66 |
| Issue | 1 | Pages | 338-45 |
| PubMed ID | 8522972 | Mgi Jnum | J:31089 |
| Mgi Id | MGI:78558 | Doi | 10.1046/j.1471-4159.1996.66010338.x |
| Citation | Karthigasan J, et al. (1996) Effects of rumpshaker mutation on CNS myelin composition and structure. J Neurochem 66(1):338-45 |
| abstractText | Myelinated CNS tissues from homozygous/hemizygous and heterozygous jimpy rumpshaker jprsh mutant mice were examined to determine the consequences on myelin structure of this mutation in the proteolipid protein (PLP) gene. Polyacrylamide gel electrophoresis and immunoblotting of brain homogenates confirmed that there was a decrease in PLP levels on the B6C3 genetic background onto which this gene was bred. We also observed an increase in level of a protein band that could correspond to the uncharacterized 10-kDa PLP previously reported in jprsh mice on an Rb(1.3) 1Bnr background. High-performance TLC and densitometry of lipids from brain homogenate and isolated myelin revealed a decrease in content of cerebrosides and sulfatides. Electron microscopy on optic nerves revealed that normal radial component is retained in jprsh myelin, further substantiating that PLP is not a component of this junctional complex. X-ray diffraction measurements on unfixed optic nerves showed that the jprsh period is 5-10 A larger than normal. Moreover, jprsh optic nerve myelin was unstable, as evidenced by a continual increase in the period postdissection. jprsh myelin that was equilibrated at varying pH and ionic strength typically had a larger than normal period under all conditions (both swelling and compacting). Our findings thus demonstrate that the biochemical abnormalities in the jprsh mutant correlate with a wider periodicity and less stable packing of the myelin. |
