| First Author | Mauldin IS | Year | 2012 |
| Journal | Blood | Volume | 119 |
| Issue | 19 | Pages | 4419-29 |
| PubMed ID | 22438258 | Mgi Jnum | J:185170 |
| Mgi Id | MGI:5427555 | Doi | 10.1182/blood-2011-09-377069 |
| Citation | Mauldin IS, et al. (2012) The tyrosine phosphatase SHP-1 dampens murine Th17 development. Blood 119(19):4419-29 |
| abstractText | Th17 cells represent a subset of CD4+ T helper cells that secrete the proinflammatory cytokine IL-17. Th17 cells have been ascribed both a beneficial role in promoting clearance of pathogenic fungi and bacteria, and a pathogenic role in autoimmune diseases. Here we identify the tyrosine phosphatase SHP-1 as a critical regulator of Th17 development, using 3 complementary approaches. Impaired SHP-1 activity through genetic deletion of SHP-1, transgenic expression of an inducible dominant negative SHP-1, or pharmacologic inhibition of SHP-1 strongly promotes the development of Th17. Ex vivo Th17 skewing assays demonstrate that genetic or pharmacologic disruption of SHP-1 activity in T cells results in a hyper-response to stimulation via IL-6 and IL-21, 2 cytokines that promote Th17 development. Mechanistically, we find that SHP-1 decreases the overall cytokine-induced phosphorylation of STAT3 in primary CD4+ T cells. These data identify SHP-1 as a key modifier of IL-6-and IL-21-driven Th17 development via regulation of STAT3 signaling and suggest SHP-1 as a potential new therapeutic target for manipulating Th17 differentiation in vivo. |
