| First Author | Tajima Y | Year | 1991 |
| Journal | Development | Volume | 113 |
| Issue | 3 | Pages | 1031-5 |
| PubMed ID | 1726563 | Mgi Jnum | J:1877 |
| Mgi Id | MGI:50401 | Doi | 10.1242/dev.113.3.1031 |
| Citation | Tajima Y, et al. (1991) Biologically active kit ligand growth factor is produced by mouse Sertoli cells and is defective in SId mutant mice. Development 113(3):1031-5 |
| abstractText | In order to define the role of Kit ligand (KL) growth factor encoded at the mouse steel (SI) locus in spermatogenesis, we have examined its production in Sertoli cells. As a measure KL growth factor bioactivity, the ability to support proliferation and maintenance of mast cells was used in co-culture with primary mouse Sertoli cells. On the sertoli cells derived from +/+ and Wv/Wv mice, +/+ mast cells proliferated and were supported for more than 2 weeks, but not W/Wv mast cells. In contrast, Sertoli cells from SId/SId mice could not support +/+ mast cell proliferation under similar conditions. The supportive effect required close-range interaction of Sertoli cells with cultured mast cells. These results indicate that Sertoli cells derived from +/+ and Wv/Wv but not SId/SId mutant mice produce biologically active KL growth factor as a membrane-bound form. The biologically active KL of Sertoli cells may also play an important role in germ cell growth and differentiation. |
